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One of many processes most more likely to be adopted is that of Dr. Caro, who converts toluene, by adding chlorine, vaporlong into benzylene dichloride, and treating the latter substance with sodium acetate, types cinnamic acid and sodium chloride. Of the opposite processes for manufacturing indigo, the primary begins from ortho-nitro-benzoic acid, which yields isatin after successive treatment with phosphorus pentachloride, silver cyanide, caustic potash, and nascent hydrogen.

The profitable experiments of this chemist had been foreshadowed by the manufacturing of alizarene, the coloring principle of the madder-root, from the anthracene of coal-tar; by the discoveries by Fritsche of the relations of indigo with the benzene ring and the amido-group; by Erdmann and Laurent's discovery that indigo on oxidation yields a crystalline body possessing no coloring power, to which they gave the name of isatin; and by Baeyer and Emmerling's accomplishment of the reverse process of reducing isatin to indigo.